superblocktm buffer (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher superblocktm buffer
Superblocktm Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbs+tween+20/Tween+20+blocking+buffer%2C+1%25+in+PBS/pm42271423-86-12-14
Average 95 stars, based on 1 article reviews

superblocktm buffer - by Bioz Stars, 2026-09

95/100 stars

Images

Incubation:

Article Title: Selective Budding of SARS-CoV-Like Particles from Glycolipid-Enriched Membrane Lipid Rafts and Host Gene Modulation.
Article Snippet: .. Following washing with PBS-Tween-20, cells were incubated for 1 h with Alexa Fluor 488-conjugated secondary antibodies (anti-human or anti-mouse IgG, Molecular Probes) diluted 1:1000 in 3% milk-PBS. ..

Article Title: A low-toxicity circular single-stranded DNA platform for safe and potent IL-12 immunotherapy against hepatocellular carcinoma.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS For immunohistochemistry, the sections were deparaffinized, rehydrated, and washed in 1% PBS-Tween-20, and then treated with 2% hydrogen peroxide, blocked with 3% goat serum and incubated for 2 h at room temperature with specific primary antibodies against CD3, CD4, CD8 and IL-12 (14-0032-82, 14-0041-82, 14-0081-82 and PA5-115401, Invitrogen, Carlsbad, CA, USA). ..

Article Title: Selective Budding of SARS-CoV-Like Particles from Glycolipid-Enriched Membrane Lipid Rafts and Host Gene Modulation
Article Snippet: .. Following washing with PBS-Tween-20, cells were incubated for 1 h with Alexa Fluor 488-conjugated secondary antibodies (anti-human or anti-mouse IgG, Molecular Probes) diluted 1:1000 in 3% milk-PBS. ..

Article Title: Thrombospondin-4 is upregulated in abdominal aortic aneurysm: A vasoprotective response with potential therapeutic relevance.
Article Snippet: .. After tissue cryosectioning, slides were air-dried under airflow for 30 min at room temperature, and then, samples were prefixed in 4% paraformaldehyde/0.1 M of PBS (pH 7.4), blocked in 2% bovine serum albumin (BSA) in PBS-Tween® 20 and treated with Image-iTTM FX Signal Enhancer (Invitrogen, Waltham, MA, United States) for 30 min. Then, the samples were incubated with an antibody against TSP4 (R&D Systems, Cat# MAB2390, RRID:AB_2202085; dilution 1:50) at room temperature for 1 h. After thorough washing, the corresponding rabbit anti-mouse Alexa Fluor 488-conjugated secondary antibody (Invitrogen) was applied. .. Afterwards, samples were washed, incubated with an antibody against CD68 (Agilent, Cat# M0876, RRID: AB_2074844) and then exposed to a chicken anti-mouse Alexa Fluor 647-conjugated secondary antibody (Invitrogen).

Article Title: Mutation of lipoprotein processing pathway gene lspA or inhibition of LspA activity by globomycin increases MRSA resistance to β-lactam antibiotics
Article Snippet: .. The secondary antibody, HRP-rec-Protein G (Invitrogen) was diluted 1:2,000 in PBS-Tween 20 (0.1%) and incubated with the membrane at room temperature for 1 h. Chemiluminescence detection was performed with the Amersham ECL Western Blotting Detection kit. .. The blot was imaged using a LICOR C-Digit Blot scanner using the Image Studio Software (version 6.1).

Article Title: Effect of different sources of selenium supplementation on immune function in pregnant and lactating ewes
Article Snippet: .. The plate was sealed and incubated at 4 °C for 46–48 h. After this incubation period, the plate was washed 5 times with 0.05% PBS-Tween-20 (Thermo Scientific Chemicals, New Jersey, USA) wash buffer using a microplate washer from BioTek Instruments (Winooski, USA). .. The plate was blocked with 200 μL of Ultrablock provided by Bio-Rad (Oxford, UK) and incubated at room temperature for 1 h. The plate was washed as described above.

Article Title: Mutation of lipoprotein processing pathway gene lspA or inhibition of LspA activity by globomycin increases MRSA resistance to β-lactam antibiotics.
Article Snippet: .. The secondary antibody, HRP-rec-Protein G (Invitrogen) was diluted 1:2,000 in PBS-Tween 20 (0.1%) and incubated with the membrane at room temperature for 1 h. Chemiluminescence detection was performed with the Amersham ECL Western Blotting Detection kit. ..

Article Title: Hospital in-use evaluation of the physical, chemical and affinity stability of the antibody-drug conjugate trastuzumab deruxtecan (Enhertu®).
Article Snippet: Wells were saturated with PBS supplemented with 3% of fetal bovine serum (FBS) for 1 h at 37 ◦C, and washed with PBS prior to incubation with T-DXd, or trastuzumab (Herzuma®, CellTrion laboratory, South Korean) from 0.001 to 1000 nM during 1 h at 37 ◦C. .. Wells were then washed with PBS-Tween 20 (0.05%) and incubated with 100 μL of protein-L-peroxydase (ThermoScientific, Pierce®, Massachusetts, USA) at 1.25 μg.mL-1 for 1 h at 37 ◦C, added to 100 μL of 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Sigma, St Louis, USA). .. Enzymatic reactions were stopped by the addition of 50 μL of 1 M H2SO4 and the absorbance was measured at 450 nm, using an absorbance microplate reader (Bio-Tek® instruments, Inc., Vermont, USA).

Review

superblocktm buffer (Thermo Fisher)

Structured Review

Images

Related Articles

Incubation:

Immunohistochemistry:

Membrane:

Western Blot:

Similar Products

Image Search Results